Many factors influence assay design, such as amplicon length, GC content, sequence specificity, and melting temperature, all of which impact the final target sequence, primers, and probes. Flawed ...

Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and ...

Exiqon is now able to address this need by offering custom assays based on LNA (Locked Nucleic Acid) enabled real-time PCR.

Applied Biosystems TaqPath assays are the first choice for probe-based qPCR assays that have been specifically designed for late-stage biotechnology and pharmaceutical applications.

The innovative LiquidArray® technology optimizes asymmetrical multiplex PCR for creating excess single-stranded amplicons with detection by Lights-On/-Off probes that contain a quencher (Lights ...

Further, they aligned primer and probe sequences and their reverse complements in the seven real-time PCR assays against 1,779 MPXV genomes and computed the percentage of genomes with a 100% match.

Custom assay design provides dPCR users with access to tailored multiplex assays for use beyond biopharma applications Milestone achieved with over 1,000 cumulative placements of the standard ...