Drosophila apple juice-agar plates - CSH Protocols
WEB1. In a conical flask, mix 18 g of agar in 600 mL of H 2 O. 2. In a separate flask, mix 20 g of sucrose in 200 mL of apple juice. 3. Place both flasks in a microwave oven on a medium setting (e.g., 7 on a scale of 0–10) for 30 min. 4.
Erlenmeyer flask - Wikipedia
WEBAn Erlenmeyer flask, also known as a conical flask (British English) [1] or a titration flask, is a type of laboratory flask with a flat bottom, a conical body, and a cylindrical neck. It is named after the German chemist Emil Erlenmeyer (1825–1909), who invented it in 1860.
WEBSterilize the conical flask or Schott bottle using a pressure cooker or autoclave 20 minutes at 1210C, 15psi, according to manufacturer’s instructions for use of this equipment. 4. The pressure in the vessel should be at zero prior to opening the equipment. The sterilized agar is allowed to cool to 500C – 550C, this is the optimal ...
Minimal media (Davis formulation) - Sharebiology
WEBTransfer the broth to conical flask or aliquot into smaller volumes. Now add agar accordingly with respect to the volume of the media (i.e., 15 gms agar for 1L of the media). Close the mouth of the flask with a cotton plug.
Conical Flask: Features, Applications, and Advantages
WEBA conical flask is a type of laboratory flask with a wide, flat bottom and an inverted cylindrical neck used in scientific work for transportation, storage, and mixing purposes. It is also commonly known as an Erlenmeyer flask or titration flask, or E-flask.
Nutrient agar and Nutrient broth - Sharebiology
WEBTransfer the broth to conical flask or aliquot into smaller volumes. If nutrient agar is required, add agar accordingly with respect to the volume of the media (i.e., 15 gms agar for 1L of the media).
WEB(e) Plug the conical flask with non-absorbent cotton. (f) Wrap the cotton plug with aluminum foil followed by pasting of indicator tape to ensure sterilization.
Microbiological Culture Media: Types, Role and Composition
WEBSep 19, 2020 · Instructions for use: Add the ingredients in 1000 ml distilled water in a conical flask. Dissolve the ingredients completely by using hot plate or suitable instrument. Sterilize the medium by autoclaving.
Sterilization of Glassware; Preparation and Sterilization of Media ...
WEBFeb 28, 2020 · Take clean, dry 500 ml of conical flask; prepare cotton plug for mouth of flask; the plug should be tight enough to restrict passage of any microbial contaminant and loose enough to allow exchange of air and moisture inside the flask.
1: Media Preparation - Biology LibreTexts
WEBMar 19, 2021 · Agar slant tubes are sterilized and then the rack is tilted to allow the agar to solidify in a slanted fashion. Agar medium to be be poured into plates is sterilized in a flask, and then poured afterward. Not all media or solutions can be sterilized via an autoclave.